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Phylogenetic analysis of plasmid pAKD4—To confirm that pAKD4 is an IncP-1 plasmid, a maximum likelihood tree was inferred from concatenated sequences of 20 backbone proteins shared by all IncP-1 plasmids and pAKD4 (Fig. 3).
Plasmid pAKD4 was previously isolated by exogenous plasmid isolation from an agricultural soil in Norway. Its 56,803bp nucleotide sequence shows high similarity in gene sequence and gene order to both plasmids pEST4011 and pIJB1, the only other IncP-1delta plasmids sequenced so far.
We conclude that pAKD4 and pIJB1 are new members of the IncP-1δ sub-group. Since pAKD4 is the only plasmid of the three with a complete set of typical IncP-1δ-like transfer genes and is self-transferable, we propose it to be the prototype plasmid for the IncP-1δ sub-group. 2. Materials and methods. 2.1.
Semantic Scholar extracted view of "Comparative genomics of pAKD4, the prototype IncP-1delta plasmid with a complete backbone." by Diya Sen et al.
Since we have already published a detailed analysis of the IncP-1δ plasmid pAKD4 , this study presents a comparative analysis of the remaining 12 pAKD plasmids and a preliminary characterization of their accessory regions. Since there are so far only 26 completely sequenced IncP-1 plasmids available to the public, this study increases this ...
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